Tag
HisTag

Part:BBa_J18909:Design

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-15)

His tag (6xHis; E. coli - optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.


There are two HIS codons: cat and cac; In E.coli, cac seems preferred among highly expressed genes during exponential phase: 70%. Metabolic proteins have about even numbers of the two codons.

Source

Synthetic DNA

References